Coeliac disease

Notes

Definition & classification

Coeliac disease is a systemic autoimmune condition triggered by dietary gluten peptides.

It is also known as gluten-sensitive enteropathy, coeliac sprue and non-tropical sprue. 

Coeliac disease is common among northern Europeans. Within the UK, the estimated prevalence is 1%. It is considered to be twice as common in females as in males.

The condition may present at any age; however a growing proportion of patients are diagnosed in older age. The presentation is varied, ranging from diarrhoea and bloating, to iron-deficiency anaemia or osteoporosis.

Aetiology

Coeliac disease develops due to intolerance to proteins called ‘prolamins’.

Prolamins are found within the seeds of cereal grains such as wheat (gliadin), barley (hordeins) and rye (secalins). They possess high proline and glutamate content which makes them resistant to digestive gastric enzymes.

Prolamins

Ingestion of prolamins can result in immune-mediated damage of the intestinal mucosa. Mucosal damage reduces in severity moving distally towards the terminal ileum.

The development of sensitivity to dietary prolamins is thought to be multifactorial, and due to a complex interaction between prolamins and the patients' genetics, immune system and the environment.

Ultimately, coeliac disease causes chronic inflammation, intestinal mucosal atrophy and malabsorption.

1. Genetics

Genetics play an important role in the development of coeliac disease. There is a strong association between coeliac disease and two type II human leukocyte antigens (HLAs):

  • HLA-DQ2 - present in 95%.
  • HLA-DQ8 - present in 5%.

HLA testing has a strong negative predictive value. This means an absence of the gene is strongly associated with not having the disease. However, having the gene does not mean patients will develop the disease (up to 25% of the general population have HLA-DQ2/HLA-DQ8).

Type II HLA genes code for proteins important in the formation of the major histocompatibility complex (MHC) class II. These are found on T helper cells and professional antigen-presenting cells (e.g. dendritic cells). They are involved in the presentation of extracellular peptides (e.g. antigens) to the immune system. They are important in the activation of the adaptive immune system (e.g. B- and T-cell-mediated immunity).

2. Immunology

Both the innate and adaptive immune systems are involved in the inappropriate response to dietary prolamins. 

Activation of  T helper cells leads to stimulation of B cells and the release of pro-inflammatory cytokines. The activation of the immune system leads to the recruitment of inflammatory cells in the lamina propria (infiltration) and subsequent hyperplasia in the crypts of Lieberkühn.

3. Environment

There is some suggestion that a number of environmental factors may be involved in the pathogenesis of coeliac disease.

Recurrent rotavirus infection infections during childhood increase the risk of developing the disease. Breastfeeding is thought to be protective.

Pathophysiology

Persistent inflammation of the intestinal mucosa leads to damage and failure of its normal absorptive functions.

Prolamins (e.g. gliadin) are ingested within the diet. These may cross the epithelial surface into the lamina propria. Here, the enzyme tissue transglutaminase is able to deaminate gliadin

Deamination makes gliadin more immunogenic and it subsequently binds to antigen-presenting cells (APCs) of the innate immune system. Gliadin is then presented to T helper cells via the HLA molecules DQ2/DQ8.

Pathogenesis of coeliac disease

Activated T helper cells secrete pro-inflammatory cytokines and activate B lymphocytes. The activation of B lymphocytes leads to the formation of auto-antibodies (e.g. anti-endomysial and anti-tissue transglutaminase).

Furthermore, gliadin may irritate endothelial cells leading to the release of cytokines (e.g. IL-15), which can activate intraepithelial lymphocytes (IEL) causing direct epithelial damage.

Collective activation of both the innate and adaptive immune responses leads to chronic inflammation and damage to enterocytes, resulting in three classical features:

  • Villous atrophy (reduced absorptive surface).
  • Crypt hyperplasia (increased cellular proliferation).
  • Inflammatory infiltration (increased IEL, influx of immune cells into lamina propria)

Clinical features

Clinical presentation is varied, ranging from diarrhoea and bloating, to iron-deficiency anaemia or osteoporosis.

Patients classically present with gastrointestinal manifestations of malabsorption including bloating, abdominal pain, loose stools and steatorrhoea.

However, some patients may present with non-specific symptoms (e.g. fatigue), whilst others may be asymptomatic. This variability and rather non-specific nature is reflected in the number of indications for testing for coeliac disease.

Symptoms

  • Weight loss
  • Fatigue
  • Weakness
  • Abdominal pain
  • Bloating
  • Flatulence
  • Loose stools
  • Steatorrhoea
  • Failure to thrive in babies and young children.

Signs

  • Mouth ulcers
  • Angular stomatitis
  • Abdominal distension
  • Ecchymosis
  • Muscle wasting
  • Neuropathy

Extra-intestinal manifestations

Extra-intestinal manifestations of coeliac disease occur as a secondary consequence of malabsorption.

Anaemia

Anaemia occurs in 10-15% of patients. Typically this is due to malabsorption of iron and folate. A such a mixed megaloblastic/microcytic picture may be seen. In severe disease affecting the ileum, B12 malabsorption may occur.

Osteoporosis

Multiple mechanisms are responsible for the osteoporosis seen in coeliac disease. Malabsorption of calcium and vitamin D deficiency contribute. Calcium may also be sequestered to poorly absorbed fatty acids.

Dermatitis herpetiformis

Dermatitis herpetiformis is a blistering skin condition that is strongly associated with coeliac disease. The rash is intensely pruritic. It may be managed with dapsone and a gluten free diet.

Other

  • Bruising: secondary to failure to absorb vitamin K.
  • Neurological sequelae: secondary to hypocalcaemia or vitamin B12 deficiency.

Investigations

Once a diagnosis of coeliac disease has been made, further investigations can be used to assess severity and monitor for the development of complications

Bedside

  • Observations
  • ECG
  • Stool culture, microscopy & sensitivities + ova, cysts & parasites.

Bloods

  • Full blood count (FBC)
  • Blood film (may reveal a variety of cellular abnormalities)
    • Microcytes (iron)
    • Macrocytes (B12/folate)
    • Hypersegmented neutrophils (B12/folate)
    • Howell-Jolly bodies (splenic atrophy)
  • Liver function tests (LFTs)
  • Urea & electrolytes (U&Es)
  • Lipids
  • Magnesium
  • Haematinics
  • Bone profile
  • Clotting
  • Serology (discussed below)
  • Genetic testing (used by specialists, high negative predictive value)

Imaging

Small bowel imaging can help to assess for complications (e.g. lymphoma). Imaging modalities include barium imaging and capsule endoscopy.

Special

Upper GI endoscopy helps to confirm the diagnosis of coeliac disease (minimum 6 biopsies). It can also be used to assess the response to treatment.

Diagnosis

Diagnosis of coeliac disease is based on both serological and histological evidence of disease activity.

Indications for testing

NICE recommends serological testing as a screen in a number of settings:

  • Based on clinical features:
    • Sudden or unexpected weight loss.
    • Chronic or intermittent diarrhoea.
    • Recurrent abdominal pain, cramping, or distension.
    • Unexplained gastrointestinal symptoms (e.g. nausea and diarrhoea).
    • Prolonged fatigue.
    • Unexplained anaemia (e.g. iron-deficiency, megaloblastic).
  • Those at increased risk:
    • Dermatitis herpetiformis
    • Autoimmune thyroid disease.
    • Irritable bowel syndrome.
    • Type 1 diabetes
    • Family history (first degree relative)
  • Other:
    • Multiple diseases associated with coeliac disease.

Diagnosis of coeliac disease

1. Serology

Patients should be tested for the presence of auto-antibodies.

There are two auto-antibodies that may be tested for:

  • IgA Anti-tissue transglutaminase (tTGA)
  • IgA Anti-endomysial (EMA)

These serological markers have a sensitivity > 90%, however, they are not always both present. Typically, anti-tTGA is assessed first. Anti-EMA should be tested for if the initial anti-tTGA test result is equivocal. 

Total IgA levels should be checked at the same time. IgA deficiency occurs in <1% of the population, but it occurs more commonly in patients with coeliac disease (2-3%). If IgA deficiency is found then tests specific for IgG anti-tTGA/anti-EMA are required.

Those with a positive serological test can be referred for intestinal biopsy and histology.

2. Histology

Patients with a positive serological test should be referred for upper GI endoscopy and intestinal biopsy

Patients may be referred for endoscopy if still highly suspicious of coeliac disease despite a negative serological test. Multiple biopsies should be taken at the time of endoscopy.

Typical histological features include:

  • Villous atrophy
  • Crypt hyperplasia
  • Inflammatory infiltration

Management

Adherence to a gluten-free diet reverses both histological and serological markers of coeliac disease.

Due to long-term complications associated with coeliac disease, it is important patients adhere to a gluten-free diet even if they do not continue to experience any symptoms. The stringency of the gluten-free diet is variable depending on the extent to which patients are affected. 

There are numerous resources and support groups available to patients to help them achieve a long-term gluten-free diet.

Complications

Two major complications include dermatitis herpetiformis and enteropathy-associated T cell lymphoma (EATCL).

Dermatitis herpetiformis

This is an IgA-mediated condition strongly associated with coeliac disease. Over 90% of patients with dermatitis herpetiformis have coeliac disease on biopsy.

Dermatitis herpetiformis is a blistering disease of the skin, which presents as an extremely itchy papulovesicular rash on the extensor surfaces. Subepidermal bullae are seen on histology with linear IgA deposits on dermal papillae. The diagnosis is based on IgA levels and skin biopsy. It is managed with dapsone.

Enteropathy-associated T-cell lymphoma (EATCL)

EATCL is an uncommon, but significant, complication of coeliac disease. It should be suspected in any patient with ‘unresponsive coeliac disease or unexplained clinical features (e.g. loss of weight). 

EATCL, a primary lymphoma of the GI tract, occurs due to chronic inflammation. It is associated with pain, obstruction, and may lead to perforation. The 5-year prognosis is poor.

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